iron nanoparticles and methionine hydroxy analogue chelate in ovo feeding of broiler chickens

the 440 fertile eggs, in a completely randomized design were divided into 11 treatments with four replicatesand 10 eggs per each replicate. treatments including: (t1; control; without injection), (t2; injected with 0.3ml saline 9.0%; sham control), (t3, t4 and t5) 25, 75, 125 ppm fe-nano, (t6, t7 and t8) 50, 100, 150 ppmfe-nano-alimet chelate, (t9, t10 and t11) 50, 100, 150 ppm fe-aliment chelate. on 10th day of incubation,0.3 ml solution in ovo was injected into the egg yolk sac. higher hatchability was found in controls, 25 ppm fe-nano, 100 ppm fe-nano-alimet chelate and all levels of fe-alimet chelate treatments than other treatments.the egg weight was higher significantly in sham control, 25 and 75 ppm fe-nano, all levels of fe-nano-alimetchelate and 150 ppm fe-aliment chelate treatments. there was significant increase in chick weight in controls,25 and 75 ppm fe-nano, 100 ppm fe-nano-alimet chelate and 150 ppm fe-aliment chelate in comparisonwith other treatments. also chick body weight to egg weight ratio in controls, 25ppm fe-nano and 100ppm fe-nano-alimet chelate was higher than in other treatments. level of 25 ppm of fe-nano has shown the highestrelative liver weight. serum fe content and liver was higher by using 25 ppm fe-nano, 100 ppm fe-nano-alimet chelate and 150 ppm fe-aliment chelate than other treatments. by applying two treatments of 100 ppmfe-nano-alimet chelate and 150 ppm fe-aliment chelate, chickens blood hemoglobin increased significantlycompared with the other treatments. these results suggest that 25 ppm fe-nano, 100 ppm fe-nano-alimetchelate and 150 ppm fe-alimet chelate improved embryonic growth and development.